Peripheral Blood As a Source of Stem Cells for Regenerative Medicine: Emphasis Towards Corneal Epithelial Reconstruction-An In Vitro Study
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Peripheral Blood As a Source of Stem Cells for Regenerative Medicine: Emphasis Towards Corneal Epithelial Reconstruction-An In Vitro Study
Background: Mesenchymal stem cell-based treatments are now emerging as a therapy for corneal epithelial damage. Although the bone marrow, adipose tissue and umbilical cord blood is a major source of mesenchymal stem cells (MSC), other tissues such as peripheral blood also harbor stem mesenchymal-like cells called the blood-derived mononuclear cells of peripheral (PBMNCs). This blood derived stem cells gained a lot of attention because of the ease of collection and isolation of minimally invasive. In this study, the feasibility of using PBMNCs as an alternative cell source for corneal limbal corneal epithelial cells regenerate stem envisaging evaluated.
Methods: Rabbits PBMNCs isolated using density gradient centrifugation and evaluated for mesenchymal cell properties including stemness. PBMNCs distinguished for corneal epithelial lineage using conditioned media limbal explants of rabbits and evaluated by immuno-cytochemistry and gene expression analysis. Furthermore, differentiated PBMNCs engineered into the cell sheet using in-house developed thermo-responsive polymer.
Results: The blood cells are derived is shown having properties similar to mesenchymal stem cells. The commitment of the corneal epithelium descendant of PBMNCs confirmed by a positive expression of CK3 / 12 markers that indicate suitability as an alternative to limbal stem cells. Resulting differentiated cells effectively in vitro cell sheets were then shown to a transfer sheet on ex vivo cells excised rabbit eyes.
Conclusion: PBMNCs as autologous cell source alternative to limbal stem cells are considered to be an effective therapeutic strategy for corneal surface reconstruction, especially for patients with bilateral limbal stem cell deficiency.
According to the investigation, people with old age and the underlying disease are at higher risk than 2019 new coronavirus infection. Indeed, they might indicate a severe form of the disease (severe acute respiratory infection). Based on the promising role of cell therapy and regenerative medicine approaches in the treatment of life-threatening diseases, it seems to apply a cell-based approach can also be a strategy hopes to raise the subject with severe acute respiratory tract infection caused by a new coronavirus 2019
Peripheral Blood As a Source of Stem Cells for Regenerative Medicine: Emphasis Towards Corneal Epithelial Reconstruction-An In Vitro Study
Adult mesenchymal stem cells and exosomes their source, characteristics and application in regenerative medicine
Human mesenchymal stem cells (MSC) has become a hot topic in the development of cell therapy and biotechnology. All types of MSC stable genome and have the capability of self-renewal. The main source of MSCs is the bone marrow, adipose tissue, umbilical cord and placenta tissue. MSCs can be cultivated in many parts differentiated to grow into cells that are more specialized, produce secretory factor and also supports trophic functions in the body.
Exosomes, derived from MSC also has great potential in regenerative medicine and tissue engineering. Exosomes secreted by MSC and has the same therapeutic potential as the parent cell. MSC and they are combined with a biomaterial exosomes can also be more effective in promoting regeneration of tissues and organs. However, for the use of MSC-exosomes as an agent distinct clinical MSC-exosomes have been produced and their effect therapy shown in clinical studies.
Description: A competitive ELISA for quantitative measurement of Canine Proteoglycan 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Proteoglycan 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Proteoglycan 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Proteoglycan 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Proteoglycan 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Proteoglycan 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Proteoglycan 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Proteoglycan 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Proteoglycan 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Proteoglycan 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Proteoglycan 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Proteoglycan 4 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
But there are still many obstacles characteristics of the unknown and still a lot needs to be conquered. In this review, we not only highlight the characteristics of human MSC and exosomes them, but also give them new therapeutic strategies in regenerative medicine.
